Authors: Elhoussieny A, El Mandooh M., Bahaa-Eldin A., Fathy H.

Abstract

To assess if laser zonal thinning prior to ICSI will improve oocyte survival, fertilization, blastocyst formation, decrease degeneration of oocytes and increase pregnancy rate or not. It was a randomized controlled trial conducted at Specialized Fertility Reproductive Science Centre (Dar El Maraa) and included 364 women who were subjected to treat their infertility by ICSI, over a 2-year period, between September 2013 and September 2015. Participants were randomly assigned following simple randomization procedures (computerized random numbers) into 2 treatment groups (conventional ICSI) control group and (laser ICSI) treatment group. Results of the trial were assisted including comparisons of oocyte fertilization and degeneration rates and blastocyst formation rates and biochemical pregnancy rate. 364 cycles were done and inseminated in accordance of the study design. The mean fertilization rate was 78.9% for C ICSI (conventional ICSI) and 75 % for LA ICSI (laser ICSI), the mean blastocyst formation rate 45.2% in C ICSI and 44.4 % for LA ICSI, the mean oocyte degeneration rate of 12.6% for C ICSI and 11.4 % for LA ICSI, mean embryo cell number on day 3 was 5.5 % for C ICSI and 5.7 % on day 3 for LA ICSI, mean degree of embryo fragmentation was 10.6% on day 3 for C ICSI and 9% on day 3 for LA ICSI. Mean pregnancy rate in C ICSI 95% and 97 % for LA ICSI with no significant difference were observed for any of the measured outcomes variables.

Keywords

Intracytoplasmic sperm injection – Laser

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Laser Assisted Zona Thinning Versus the Conventional Mechanical Method for Intracytoplasmic Sperm Injection in ICSI Programs